The automatic polarimeter is an instrument for measuring the rotatory power of matter. Through the rotation of the sample determination, analysis can determine the concentration, content and purity of material. Automatic indexing device of photoelectric detector and electronic automatic polarimeter, has the advantages of small size, high sensitivity, no difference, characteristics of easy reading. The low rotation sample can also be adapted to the visual polarimeter. Therefore, it is widely used in medicine, food, organic chemical and other fields.
Agriculture: analysis of agricultural antibiotics, agricultural hormones, microbial pesticides and agricultural products.
Medicine: antibiotics, vitamins, glucose and other drug analysis, pharmacological studies of Chinese herbal medicine.
Food: sugar, monosodium glutamate, soy sauce production process control and finished product inspection, food sugar content determination.
Petroleum: analysis of mineral oil and monitoring of petroleum fermentation process.
Spices: analysis of essential oils.
Health care: clinical diabetes analysis in hospital.
First, operation method
1 insert the power plug of the instrument into the 220V AC power supply, use the AC electronic voltage regulator (1KVA) and reliably connect the grounding pin.
2 open the power switch, need to be 5min sodium lamp preheating, so that the luminous stability.
3 turn on the DC switch (if the DC switch is switched on and the sodium lamp is extinguished, then the DC switch is repeated 1 to 2 times, so that the sodium lamp is lit under the direct current, which is normal)
4 open the display switch, adjust the zero handwheel, so that the optical indication is zero.
5 put the test tube containing distilled water or other blank solvents into the sample room and cover the box cover. If there is a bubble in the test tube, the bubble should first float on the convex neck, and the fog droplets at both ends of the smooth surface. Apply soft cloth to dry. Test tube nut should not be too tight, so as not to produce stress, affect reading. Attention should be paid to the location and direction of the marker in the test tube placement.
6 take out the test tube. The sample is injected into the test tube and put into the sample room in the same position and direction, and then the cover is covered. The dial will turn out the optical rotation of the sample. The red value on the dial is left (-) black, indicating the right (+).
7 successive press test button, repeat several times, the average value is taken as the determination results of samples.
8 if the sample exceeds the measuring range, the instrument stops automatically at + 45. At this time, take out the tube, press the reset button switch, the instrument automatically return to zero.
9 after the instrument is used, the indicator, DC and power switch should be closed in turn.
When the 10 sodium lamp fails to be used in the DC power supply system, the instrument can also be tested in the case of sodium lamp alternating current power supply, but the performance of the instrument may be slightly reduced.
Two. Determination of concentration or content
First, the standard sample or reference sample of known purity was released into several samples with different concentration according to a certain proportion, and then their rotatory power was measured. Then to the horizontal axis of the vertical axis for rotation, concentration, rotation curve plotted. In general, the optical rotation curves are checked by the arithmetic interpolation method.
When measuring, the optical rotation of the sample is first measured, and the concentration or content of the sample is determined from the optical rotation curve according to the rotation.
The optical rotation curve should be used in the same instrument and in the same test tube, so attention should be paid to the determination of the optical rotation curve.